The hybcell – and therefore the array itself – is constantly moved (rotated) through the sample. This means that the sample is rapidly and homogeneously agitated around the probes coated on the surface, resulting in improved reproducibility, drastic reduction in reaction times, and flexible assay formats (e.g. a sequence of scans and heating and/or cooling steps).
After common hybridization of DNA probes takes place on the microarray’s surface, compact sequencing continues with an enzymatic reaction on the spots (solid phase primer extension), followed by a stringent washing step. The intention of compact sequencing is the differentiation of target DNA down to a single base.